4 Mistakes You’re Making in Solid Phase Extraction

Separation techniques are vital in the scientific world to develop and analyze compounds. Separation can happen in several ways, including extraction techniques such as liquid-liquid extraction and supported liquid extraction, as well as one of the most popular, solid-phase extraction. In any extraction technique, the smallest mistake can lead to drastically inaccurate results. In this guide, we’ll discuss four of the most common mistakes you’re making in solid-phase extraction.

Using the Wrong Sorbent

As extraction methods continue to advance, more and more sorbent materials are available to choose from. However, the abundance of options can increase your chances of choosing the wrong one. In many cases, the extraction you are performing will require a specific sorbent, but some offer a wide range of options. In these instances, the sorbent must always tailor to the analytes in the extraction.

Not Considering Chromatography

One should always treat solid-phase extraction like a chromatography technique. Both separation methods have similar goals and retention methods, although chromatography allows for more selective retention. If you treat your extraction technique as a form of chromatography, you can optimize retention in a more particular manner.

Ignoring the Matrix

While much of your concern might be on the analyte(s) separation, you need to keep the entire sample in mind. For example, the medium you use to conduct the extraction must be able to withstand the matrix as well. If your sample matrix’s chemical capacity is higher than the medium of choice, your extraction will not be successful.

Skipping Solvent Drying

Solvent drying is a necessary step in solvent evaporation. It removes excess water so that the evaporator can reduce the extract volume to an appropriate analysis level. Skipping the solvent-drying phase will result in extra water in your results, altering the accuracy. Extraction of any kind is a tedious process, especially solid-phase extraction. Avoid these four mistakes you’re making in solid-phase extraction to make sure you get accurate results.